Notably the development of replicating competent reporter expressing viruses rcrevs has provided an excellent option to detect directly viral replication without the use of secondary labeling which represents a significant advance in virology. These viruses have been employed for in vitro and in vivo studies such as the screening of neutralizing antibodies or antiviral compounds the identification of host factors involved in viral replication cell tropism the development of vaccines or the assessment of viral infection dynamics in summary reporter expressing replicating competent iav represent a powerful tool for the study of iav both in vitro and in vivo. Studying viral dissemination in vivo using replication competent reporter expressing iav remains a challenge because it requires that the reporter signal to be detected through tissue and skin pan et al published the generation of replication competent iav harboring gluc in the na segment using pr8 as the backbone iav gluc the viral na segment was similar to the described above for rpr8 na egfp iav gluc replicated at lower levels in mdck cells 2 3 log and eggs 1 2 log as . Stable incorporation of foreign genes in replication competent reporter expressing iav allows for effective tracking of viral infection in vitro and in vivo enabling a robust quantitative readout this readout can be used with high throughput screenings hts and to assess viral infection in tissue
How it works:
1. Register Trial Account.
2. Download The Books as you like ( Personal use )